AMD 疾病机制与新药优化方向博士职位

Assess the involvement of Factor H-related proteins (FHRs) accumulation on ECM stability and endothelial cell homeostasis The PhD position is part of the MSCA Doctoral Network PANDORA on discovering Pathomechanisms and Novel Drug Optimization for AMD (Age-Related Macular Degeneration). AMD is the most common cause of blindness in the elderly population, and its pathological mechanisms are complex and not fully understood. In human patients, the elevated systemic levels of five factor H-related proteins (FHRs1-5) have been associated with the initial stages of AMD progression. FHRs accumulate in the blood vasculature underlying the outer blood/retinal barrier and are believed to interfere with the regulatory functions of complement factor H, which protects extracellular matrices (ECM) from aberrant complement activation, inflammation and tissue remodeling. However, the molecular consequences of FHR protein accumulation on the choroidal endothelial cells (EC) and its effects on cellular homeostasis, maintenance of the ECM, and the subsequent effects on the associated retinal pigment epithelial (RPE) cells are not yet known. The successful candidate will elucidate the effects of FHR protein accumulation in the outer blood/retinal barrier and its contribution to disease pathology. The generation of highly characterised in-house recombinant proteins is a valuable tool in investigating pathological mechanisms and disease. The successful candidate will create a library of recombinant FHR proteins and further optimize our in-house cellular models of the outer blood/retinal barrier. Together, this will allow the direct exposure of the model to varying FHR protein concentrations representing those found in the blood of healthy and AMD patients. The effects on gene transcription, protein expression, cell morphology, ECM composition, and functional readouts such as diffusion and angiogenesis will all be explored. The model will provide a basis for the potential testing of future therapeutic strategies. We are seeking a highly motivated and intellectually curious PhD student, ideally with strong background in cell culture and biochemistry. The candidate will investigate the biochemistry driving AMD. The project aims to dissect AMD profiles depending on genetic susceptibility, stressors, and the contribution of the complement system. The PhD candidate will benefit from a network of European experts in different fields (gene therapy, pharmacology, bioinformatics, disease modeling, imaging) and will have the possibility to visit other labs in secondments (lab exchanges). The doctoral candidate will utilize cutting-edge techniques: • Cell and tissue culture (human primary and stem cell-derived retinal models) • Molecular biology and biochemistry techniques (qPCR, immunohistochemistry, western blot) • Cell analyses (in vitro assays, metabolism measurements)

Recent open access research publications related to this position: https://onlinelibrary.wiley.com/doi/10.1002/eji.70228 https://www.nature.com/articles/s41467-020-14499-3 https://www.sciencedirect.com/science/article/pii/S000292972100224X?via%3Dihub https://www.sciencedirect.com/science/article/pii/S0002929721002202?via%3Dihub

Requirements / required education level / degree: Master Degree or equivalent

Requirements / required education level / discipline: Biological sciences

Requirements / required education level / degree: Master Degree or equivalent

Requirements / required education level / discipline: Neurosciences

Requirements / required education level / degree: Master Degree or equivalent

Requirements / required education level / discipline: Medical sciences

Requirements / skills: • A Master’s degree in life sciences (biology, neurobiology, bioinformatics or closely related fields) • Good background in cell biology and/or biochemistry • Excellence in oral and written English, with good presentation skills • High motivation with interest in retinal degeneration and disease mechanisms • Work effectively both independently and as part of a team • Knowledge of cellular and molecular biology, eye biology and/or neuroscience • Strong interest in translational biomedical research

Requirements / specific requirements: • Hands on knowledge in cell culture, molecular biology and biochemistry techniques • Background in complement system biology is a plus

Requirements / required languages / language: ENGLISH

Requirements / required languages / language level: Excellent

Additional information / benefits: The EU provides 36 months support for each recruited researcher. We offer a highly competitive salary, with a gross salary in the range of 3.924 € (before taxes and employees' social security deductions). The Institute for Ophthalmic Research is a vibrant academic environment, hosting 13 research labs and 2 clinical research labs, and a core facility for Medical Proteomics. The institution is renowned as a leading centre for vision research. As an integral part of Tübingen’s biomedical and neuroscience campus, we offer a scientific environment that favors creativity for generating groundbreaking ideas, their transfer into reality and their translation into diagnostics and therapy to help those that suffer from vision impairment. The candidate will have the opportunity to interact with other labs and will get exposure to an array of techniques relevant to disease modeling and therapeutics. We offer modern, state-of the art laboratories, facilities, and equipment, as well as participation in training activities of the international and intersectorial PANDORA network. We also promote participation in national and international conferences.

Additional information / eligibility criteria: The applicant • shall not have resided or carried out his/her main activity (work, studies etc.) in Germany for more than 12 months in the 36 months immediately before the recruitment date (unless as part of a compulsory national service or a procedure for obtaining refugee status under the Geneva Convention). • shall not already be in possession of a doctoral degree (*). • can be of any nationality.

*researchers who have successfully defended their doctoral thesis but who have not yet formally been awarded the doctoral degree will not be considered eligible

Additional information / selection process: Doctoral candidates will be selected based on scientific qualification and experience, research interest, additional knowledge/skills and their motivation to participate in an intersectoral research-training program.

Additional information / comment: Please send your CV, specifying the title of the position, a 1-page cover letter and the contact details of one or two academic referees to: Pamela De Fino pamela.defino@med.uni-tuebingen.de If you have informal enquiries- please contact Prof. Simon Clark simon.clark@uni-tuebingen.de

Work location / nr job positions: 1

Work location / job organisation institute: Institute for Ophthalmic Research, University of Tübingen

Work location / job country: Germany

Work location / job city: Tuebingen

Work location / job postal code: 72076

Work location / job street: Elfried-Aulhorn-Str. 7

Hiring contact / organisation institute: Institute for Ophthalmic Research

Hiring contact / organisation institute type: Public Research Institution

Hiring contact / division faculty: Ophthalmology

Hiring contact / country: Germany

Hiring contact / city: Tübingen

Hiring contact / postal code: 72076

Hiring contact / street: Elfriede-Aulhorn Str. 7

Hiring contact / e mail: pamela.defino@med.uni-tuebingen.de

Hiring contact / e mail: simon.clark@uni-tuebingen.de

Hiring contact / website: https://www.medizin.uni-tuebingen.de/de/das-klinikum/einrichtungen/institute/forschungsinstitut-augenheilkunde

Hiring contact / website: https://www.eye-tuebingen.de/

Hiring contact / phone: 004970712987892

Application / how to apply: e-mail

Application / application email: pamela.defino@med.uni-tuebingen.de

EU funding / framework programme: Horizon Europe - MSCA

EU funding / cofund nr job position: 1

EU funding / sesam agreement number: 101312030

Research field / main research field: Biological sciences

Research field / sub research field: Biology

Research field / main research field: Neurosciences

Research field / sub research field: Neurobiology

Research field / main research field: Medical sciences

Research field / sub research field: Health sciences

Researcher profile: First Stage Researcher (R1)

Positions: PhD Positions

Contract: Temporary

Job status: Full-time

Application deadline (as exported; timezone unverified): 2026-10-15T21:59:59