利用单细胞多组学揭示增强子对血液与心血管命运调控的博士研究员
PhD Studentship: One Cell at a Time: Uncovering Enhancer Control of Blood and Cardiovascular Fate Using Single-cell Multi-omics
原帖优先：申请材料、截止时间与资格以原帖和学校官方说明为准。
AI 中文速览
- 研究内容
- 本项目将利用单细胞多组学（结合基因表达与染色质可及性数据），研究鸡胚中血液、内皮和心脏谱系分化时增强子活性的变化。你将学习计算生物学方法整合单细胞RNA测序和ATAC测序数据、重建基因调控网络，并通过实验将候选增强子克隆到荧光报告构建体中，在活体胚胎中可视化其活性，同时利用基于CRISPR的干扰和基序突变在体内测试选择性增强子如何控制基因表达、细胞行为和命运决定。
- 申请条件
- 原文未说明具体的学位和背景专业硬性要求。
- 待遇
- 提供为期4年的Norwich Research Park Biosciences Doctoral Training Partnership (NRP DTP) 博士生奖学金，包含学费支付、生活费津贴（2026/27年度津贴标准为每年21,805英镑）以及每年5,000英镑的研究培训支持补助金（RTSG）。
- 申请方式
- 请点击上方的“Apply”按钮进行申请；如需进一步信息，可联系 g.mok@uea.ac.uk。截止日期为2026年11月30日23:59。
由 gemini-2.5-flash-lite 生成，博士岗判定置信度 100%。
岗位信息
- 截止
- （Europe/London） 剩 37 天
- 学科
- 生命科学
- 合同类型
- 雇佣合同
- 原文薪资
- GBP 21,805 / 年（税前）
- 税后月薪（估）
- ¥13,700；房租后 ¥5,400
- 估算假设
- 单身、无子女、雇佣合同的粗略估算，以学校 offer 为准；扣除率 18%；汇率日期 2026-10-01
- 原帖发布
- 本站收录
- 内容更新
- 原帖导师候选（待核验）
- Dr Gi Fay Mok, Professor Andrea Münsterberg, Dr Iain Macaulay, Dr Wilfried Haerty
- 来源
- jobs.ac.uk（英国博士项目及学术招聘） · 最近核对 2026-10-23
- 详情核验
判定依据（原文摘录）
- 这是一项博士培养机会
This project is awarded with a 4-year Norwich Research Park Biosciences Doctoral Training Partnership PhD DTP studentship.
原文
The cardiovascular system - comprising the heart, blood and blood vessels - is the first functional organ system to form during vertebrate development. Cardiac progenitor cells generate the heart, while hematoendothelial progenitors produce blood and vascular endothelium. Although these lineages share an origin in the lateral plate mesoderm, they acquire distinct identities through tightly controlled gene-regulatory programmes. Many transcription factors involved in cardiovascular development are known, but much less is understood about the enhancers that control when and where these genes are activated. Enhancers are non-coding DNA regions that direct cell-type-specific gene expression. Their disruption is increasingly associated with congenital cardiovascular and blood disorders yet identifying functional enhancers and testing their roles in the developing embryo remain major challenges.
In this PhD, you will use single-cell multi-omics, combining gene-expression and chromatin-accessibility data, to investigate how enhancer activity changes as blood, endothelial and cardiac lineages emerge in the chick embryo. You will learn computational approaches to integrate single-cell RNA-sequencing and ATAC-sequencing data, reconstruct gene-regulatory networks and identify candidate enhancers linked to specific cell states and developmental trajectories. Particular emphasis will be placed on how hematoendothelial progenitors generate the first blood and vascular cells, with cardiac development providing an important comparison. Experimentally, you will clone candidate enhancers into fluorescent reporter constructs and visualise their activity in living embryos. CRISPR-based perturbation and motif mutagenesis will then test how selected enhancers control gene expression, cell behaviour and fate decisions in vivo.
You will join a collaborative supervisory team across UEA and the Earlham Institute, led by Dr Gi Fay Mok with Professor Andrea Münsterberg, Dr Iain Macaulay and Dr Wilfried Haerty. The project provides interdisciplinary training in developmental biology, molecular genetics, imaging, single-cell genomics and computational biology.
For further information, contact g.mok@uea.ac.uk .
Funding Details
This project is awarded with a 4-year Norwich Research Park Biosciences Doctoral Training Partnership PhD DTP studentship. The studentship includes payment of tuition fees (directly to the University), a stipend to cover living expenses (2026/27 stipend rate: £21,805, and a Research Training Support Grant of £5,000pa for each year of the studentship.
For further information please click on the ' Apply ' button above.
Closing Date: 23:59 on 30 November 2026.